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991.
992.
There are rising concerns about the hazardous effects of heavy metals on the environment. In this study, comet assay and DNA alkaline unwinding assay were conducted on the tissues (gills, hepatopancreas, and hemocytes) of Charybdis japonica in order to illustrate genotoxicity of three heavy metal ions (Cu2+, Pb2+, and Cd2+) on the marine crabs C. japonica. The crabs were exposed to Cu2+ (10, 50, and 100 ?g.L?1), Pb2+ (50, 250, and 500 ?g L?1) and Cd2+ (5, 25, and 50 ?g L?1), and the tissues were sampled at days 0.5, 1, 3, 6, 9, and 15. DNA alkaline unwinding assay was used for testing the DNA single strand break in gills and hepatopancreas and comet assay was employed for testing the DNA damage in hemocytes. The results showed that the DNA damage (F-value) of gills in the crabs exposed to the three heavy metals was decreased gradually during the exposure periods and there was a dose-time response relationship in certain time, suggesting that the levels of DNA single strand break in all the experimental groups increased significantly compared to the controls. Changes of F-value in hepatopancreas of the crabs exposed to the three heavy metals were similar to those in gills except that the peak values were found in the 500 ?g L?1 Pb2+ treatment group at day 3 and the 50 ?g L?1 Cd2+ treatment group at day 9. The ranks of DNA damage in gills and hepatopancreas induced by the three heavy metal ions (50 ?g L?1, day 15) were Cd2+ >Pb2+ >Cu2+ and Pb2+ >Cu2+ >Cd2+. The levels of DNA damage in gills were higher than those in hepatopancreas in the same experimental group. It can be concluded that indices of DNA damage can be used as the potential biomarkers of heavy metal pollution in marine environment. 相似文献
993.
994.
南大巴山西北段镇巴—下高川地区地质构造解析 总被引:1,自引:0,他引:1
在南大巴山西北段镇巴—下高川地区进行1∶50 000地质填图基础上,通过对地质构造现象野外的详细观测,运用持平投影的构造几何学和运动学等方法对主要断层面擦痕和断层两侧不对称褶皱枢纽以及褶皱两翼产状进行统计分析和构造解析,结合区域沉积资料和地质年代学资料,对该区构造进行构造序列分析,并探讨南大巴山构造带的变形时限、动力学机制及其演化。研究表明:南大巴山西北段镇巴—下高川地区断裂构造特征表现为高角度逆冲推覆兼有右行走滑性质的叠瓦式逆冲推覆构造;与逆冲推覆构造相关褶皱多为轴面东倾西倒的同斜褶皱和斜歪褶皱,而且由东向西褶皱紧闭程度逐渐减弱,表明逆冲推覆方向由东向西,扩展方式为前展式。该区是在印支期扬子地块与秦岭造山带全面碰撞造山作用基础上,在燕山期中—晚期整个大巴山构造系由北东向南西方向大规模的陆内造山,形成了现今的构造几何形态,其中右行走滑构造是燕山期中—晚期(J3-K2)逆冲推覆构造持续向西南推移过程中形成的。 相似文献
995.
996.
眼柄粗提物对凡纳滨对虾性分化的影响 总被引:1,自引:0,他引:1
采用生化方法制备凡纳滨对虾[Litopenaeus(Penaeus)vannamei)]眼柄粗提物,用活体注射法研究不同粗提物剂量对去眼柄凡纳滨对虾卵母细胞直径大小的影响,以检验其性腺抑制素(Gonadinhibiting hormone,GIH)的生物活性;在凡纳滨对虾仔虾后17、22和27 d,分别以不同浓度眼柄粗提物浸泡24 h,结果显示:除0.001个眼柄/mL的GIH浓度外,0.01、0.1和1个眼柄/mL组均显著降低凡纳滨对虾卵母细胞直径(P﹤0.05);浸泡处理后,仔虾后17 d GIH处理组凡纳滨对虾雌性率达70.1%~80.1%;仔虾后22 d GIH处理组雌性率达65.8%~71.7%,各浓度GIH处理组雌性率比对照组(雌雄比例约为1:1)显著提高(P﹤0.05);仔虾后27 d,0.01和1个眼柄/mL处理组雌性率(约58%)略高于对照组,但0.1个眼柄/mL处理组雌性率(73.1%)与对照组相比显著提高(P﹤0.05)。结果表明较低浓度的眼柄粗提物可明显诱导凡纳滨对虾雌性化,在仔虾后22 d前处理效果最佳。 相似文献
997.
以国内外20篇海事声明作为语料,运用语料库分析软件进行体裁分析,归纳出海事声明体裁六大语步的功能和语步结构;并对其语言特点进行分析和解读,以加深从业人员对海事声明体裁的理解,熟练拟写海事声明。 相似文献
998.
Finite element analysis on impact of foundation treatment in bridgehead transition section upon bridge piles 下载免费PDF全文
In order to decrease relative settlement, foundation treatment plays an extremely important role in bridgehead transition section, especially, the situation of building the bridge piles firstly, and th... 相似文献
999.
Daniel Brooker 《Singapore journal of tropical geography》2011,32(2):141-154
The paper seeks to illuminate everyday work practices in the economic spaces of Malaysia's business process outsourcing (BPO) industry. It draws upon empirical evidence from call centre staff and promotes analytical sensitivity to the experience of labour and spatiotemporal relations within the offshore back offices of multinational corporations located in Cyberjaya, Malaysia. The analysis provides an empirical snapshot of how IT‐facilitated work practices in the global information economy are embedded and socially reproduced in everyday settings. To this end, the paper engages anthropologically with BPO labour, and critiques ‘globalist’ representations of economic and social transformation frequently articulated through an abstract, dehumanized space of networks and flows often seen to wash over local places, culture and people. 相似文献
1000.
Improving household accessibility to basic community services can help reduce poverty in upland areas. In this study, spatial analysis with GIS was used to measure the accessibility of different household income groups to community services in the landlocked upland municipality Claveria in Northern Mindanao, the Philippines. Important community services were identified through villagers' participation in a matrix‐scoring activity. Travel information was derived from key informant interviews while the geographical coordinates of sample households and important services were collected using GPS receivers. The Flowmap GIS software (version 7.2) was used to compute accessibility to services along the road network by habal‐habal (two‐wheel motorcycle). Outcomes from the participatory data gathering activities revealed that agricultural, educational and health facilities, as well as government services are important to the community to achieve sustainable livelihoods. Because local people perceive accessibility in terms of monetary costs, rather than distance, road distance measurements were converted into fare costs. Results of the accessibility analysis show that higher‐income household groups generally incur lower mean one‐way travel costs to reach important community services than poorer households. However, almost all households spend more than the daily per capita poverty threshold for the province to reach basic community services. A scenario to improve accessibility to services in the study area was investigated to emphasize the potential of GIS‐based accessibility analysis in rural service planning. 相似文献